منابع مشابه
PCR-based multiparametric assays in single cells.
Pathologists have long known that the functional state of a tissue is best understood from an overview of the different cells making up that tissue— cells that may differ radically in their lineage, their phase in the cell cycle, their responses to stimuli, and their clonal history of somatic mutation, e.g., in a tumor. With the radically expanding opportunities for the molecular analysis of nu...
متن کاملValidation of Quantitative PCR Assays
can assess the safety of biotechnology products. Q-PCR precisely quantifies the amount of the nucleic acid target sequence in DNA or RNA extracted from a variety of samples including animal tissues, final products, cell banks, chromatography eluates, and bulk harvest material. During amplification with target-specific oligonucleotide primers, a fluorogenic oligonucleotide probe — with both repo...
متن کاملCalibration of Quantitative PCR Assays
Quantitative real-time PCR (polymerase chain reaction) assays are increasingly used to measure quantities of nucleic acids in samples. They may be used to provide a high-throughput alternative to more traditional biological assays. In this case, a calibration process may be required to convert the PCR measurements onto a more relevant scale. This is most commonly undertaken using simple linear ...
متن کاملmethGraph: a genome visualization tool for PCR-based methylation assays.
Abnormalities in DNA methylation of CpG islands that play a role in gene regulation affect gene expression and hence play a role in disease, including cancer. Bisulfite-based DNA methylation analysis methods such as methylation-specific PCR (MSP) and bisulfite sequencing (BiSeq) are most commonly used to study gene-specific DNA methylation. Assessing specificity and visualizing the position of ...
متن کاملOptimizing PCR Assays for DNA Based Cancer Diagnostics
Somatically acquired DNA rearrangements are characteristic of many cancers. The use of these mutations as diagnostic markers is challenging, because tumor cells are frequently admixed with normal cells, particularly in early stage tumor samples, and thus the samples contain a high background of normal DNA. Detection is further confounded by the fact that the rearrangement boundaries are not con...
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ژورنال
عنوان ژورنال: Immunohematology
سال: 2020
ISSN: 0894-203X,1930-3955
DOI: 10.21307/immunohematology-2019-294